Another interesting study called "adenovirus-mediated Bak gene transfer causes apoptosis in mesothelioma cell lines" by Abujiang Pataer, MD, PhD, W. Roy Smythe, Dr. Robert Yu, MS, Bingliang Fang, MD, PhD . sc, Tim McDonnell, MD, PhD, Jack A. Roth, Dr. Stephen G. Swisher, MD - from the Department of Thoracic Molecular Oncology, Department of Thoracic and cardiovascular surgery, and Institute of Molecular Pathology, University of Texas MD. Anderson Cancer Center, Houston, Texas - General Thoracic Surgery, J Thorac Cardiovasc Surg 2001; 121:0061-0067 Here is an excerpt: "Objective: Conventional treatment for mesothelioma is largely ineffective, therefore, evaluated the new approach. By adenoviral gene transfer of proapoptotic Bcl-2 family Bak in mesothelioma cancer cell lines, which are sensitive and resistant to adenoviral p53.
Methods: Binary adenoviral Bak (Ad / GT-Bak and Ad/GV16) and Laczi (Ad / GT-Laczi and Ad/GV16) vectors were used to convert from mesothelioma cell lines I-45 (p53-resistant ) and REN (p53-sensitive). Protein levels are determined by Western blotting. Apoptosis was assessed morphological changes, caspase-3 cleavage, and fluorescence-activated cell sorting analysis of subdiploid populations. Cell viability was determined by XTT assay. Statistical analysis was performed with analysis of variance and Student test.
Methods: Binary adenoviral Bak (Ad / GT-Bak and Ad/GV16) and Laczi (Ad / GT-Laczi and Ad/GV16) vectors were used to convert from mesothelioma cell lines I-45 (p53-resistant ) and REN (p53-sensitive). Protein levels are determined by Western blotting. Apoptosis was assessed morphological changes, caspase-3 cleavage, and fluorescence-activated cell sorting analysis of subdiploid populations. Cell viability was determined by XTT assay. Statistical analysis was performed with analysis of variance and Student test.
...Methods: Binary adenoviral Bak (Ad / GT-Bak and Ad/GV16) and Laczi (Ad / GT-Laczi and Ad/GV16) vectors were used to convert from mesothelioma cell lines I-45 (p53-resistant ) and REN (p53-sensitive). Protein levels are determined by Western blotting. Apoptosis was assessed morphological changes, caspase-3 cleavage, and fluorescence-activated cell sorting analysis of subdiploid populations. Cell viability was determined by XTT assay. Statistical analysis was performed with analysis of variance and Student test.
...Methods: Binary adenoviral Bak (Ad / GT-Bak and Ad/GV16) and Laczi (Ad / GT-Laczi and Ad/GV16) vectors were used to convert from mesothelioma cell lines I-45 (p53-resistant ) and REN (p53-sensitive). Protein levels are determined by Western blotting. Apoptosis was assessed morphological changes, caspase-3 cleavage, and fluorescence-activated cell sorting analysis of subdiploid populations. Cell viability was determined by XTT assay. Statistical analysis was performed with analysis of variance and Student test.
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